The gpx4 activity assay kit
WebInhibition of GPX4 can induce cell death by ferroptosis (see, Yang, W. S., et al. Regulation of ferroptotic cancer cell death by GPX4. Cell 156, 317-331 (2014)). Inhibitors of GPX4 include GPX4-binding proteins (e.g., antibodies or antibody fragments), nucleic acid inhibitors (e.g., antisense oligonucleotides or siRNAs), and small molecules that specifically inhibit GPX4. Web10 Nov 2024 · The levels of reactive oxygen species (ROS) and lipid reactive oxygen species (Lipid ROS) were detected by fluorescent probes Dihydroethidium (DHE) and C11 BODIPY 581/591. Iron Colorimetric Assay Kit, malondialdehyde (MDA) and glutathione (GSH) activity assay kits were used to detect intracellular iron ion, MDA and GSHcontent.
The gpx4 activity assay kit
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WebMetabolism Assay Kits Description General description Glucose 6-phosphate (G6P) is a key metabolic intermediate that enters either metabolic pathways or storage. G6P is generated when glucose is phosphorylated by hexokinase or glucokinase or by the conversion of glucose-1-phosphate by phosphoglucomutase during glycogenolysis. WebGPX1 and GPX4 Enzyme Activity. After being cultured under various experimental conditions, the cells were washed, scraped on ice; around 100 mg tissues were flash-freezed in liquid nitrogen. ... An aspartate aminotransferase (AST) assay kit, an alanine aminotransferase (ALT) assay kit and a homocysteine assay kit from Nanjing Jiancheng ...
WebGPX4 activity was analyzed using a glutathione peroxidase 4 assay kit (Abcam, Cambridge, MA, USA). The kit uses a chromogenic reagent that reacts with the lipid peroxidation … WebGPX4-Specific Activity Assay Glutathione peroxidase 4-specific activity was measured in GPX4 mouse embryonic fibroblasts (MEFs). GPX4 specific activity was carried out in a …
WebELISA Kit for Glutathione Peroxidase 4 (GPX4) Enzyme-linked immunosorbent assay for Antigen Detection. LMC994Hu: Magnetic Luminex Assay Kit for Glutathione Peroxidase 4 … WebThe total NO content was evaluated using a nitrate-nitrite assay kit (Nanjing Jiancheng, China) according to the standard protocol. An ELISA kit (Nanjing Jiancheng, China) was applied to measure cGMP levels as described. ... Thus, GPX4 activity is critical for maintaining lipid homeostasis in cells, preventing the accumulation of toxic lipid ...
Web3 Oct 2024 · evaluate the effects of compound on GPX4 activity, compound was first pre-incubated with purified GPX4 in the assay buffer [Tris-HCl (100 mM, pH 7.4), EDT A (5 …
Web10 Apr 2024 · For chronic liver injury, controls were perfused with 0.2 mL distilled water for 4 weeks and treated with corn oil (with or without 10% CCl 4, see above) at d 1, 3 and 5 of the week.The mice were given 100 and 200 mg/kg MFAEs orally for 4 weeks followed with 10% CCl 4 in low-dose and high-dose MFAEs groups [].Positive drug group were given 200 … bugaboo stardust pop-up playardWeb12 Apr 2024 · The protein concentration was detected by BCA protein assay kit (P0012, Beyotime, Shanghai, China). Next, the levels of CLDN5, 4-HNE, GPX4 and xCT were analyzed by Western blotting. Equal amounts of denatured protein (30 ng per lane) were separated in 8%–12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), … crosby cad filesWebProduct Details GPX4 ELISA Kit (hide) For the quantitative determination of mouse phospholipid hydroperoxide glutathione peroxidase, mitochondrial (GPX4) concentrations … crosby cage code