WebVector type Mammalian Expression Growth in Bacteria Bacterial Resistance (s) Kanamycin, 50 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number High Copy Gene/Insert Gene/Insert name GFP Species Synthetic Insert Size (bp) 720 Promoter CMV Cloning Information Cloning method Ligation Independent Cloning WebWe looked at pIRES expression vectors (Clontech) with a reporter (GFP) cloned in either the Multiple Cloning Site (MCS) upstream of the IRES (con - struct A) or downstream of the IRES (construct B; Figure 2A). Figure 2B demonstrates that GFP expression is drastically reduced if GFP is located downstream of the IRES.

pMS4 (Empty insertion vector with SEC) - Addgene

WebMar 25, 2004 · Green fluorescent protein (GFP) has been used as a reporter molecule for gene expression because it emits green fluorescence after blue-light excitation. 1, 2 Inclusion of this gene in a vector... WebCycle 3 GFP fluorescence can be detected using a filter set designed to detect wild-type GFP (since they have the same fluorescence spectra). In-house, we use the XF76 filter set from Omega Optical. For Cycle 3 GFP, excite at 395 nm and read emission at 507 nm. You can also look at the emission spectra and record emissions from 200–800 nm. milchhof handorf

Addgene: Vector Database - pMK4

WebAug 25, 2024 · The GV3101 strains containing different vectors were then separately co-infiltrated with green fluorescent protein (GFP) expression vector pMS4 into the N. benthamiana leaves. The function of VSRs was examined by photographing under UV four days after infiltration (DAI). WebPlasmid pMXs-puro Vps34-GFP from Dr. Noboru Mizushima's lab contains the insert Phosphatidylinositol 3-kinase catalytic subunit type 3 and is published in Mol Biol Cell. … WebPlasmid pMS4 (Empty insertion vector with SEC) from Dr. Patrick Phillips's lab contains the insert SEC and is published in G3 (Bethesda). 2024 Aug 19. pii: g3.120.401400. doi: … new years 2003