Webb10 jan. 2024 · Construction of the Deletion Mutant. The coding region of the mcr-1 gene was deleted from E. coli K12 BW25113 carrying plasmid pHNSHP45 (Liu et al., 2016) following a one-step inactivation method (Datsenko and Wanner, 2000).The primers used in this study are shown in Supplementary Table S1.PCR products containing … Webb8 jan. 2016 · [Source: The Lancet Infectious Diseases, full page: (LINK). Summary.] Correspondence Colistin resistance gene mcr-1 and pHNSHP45 plasmid in human …
Associations between human bacterial pathogens and ARGs are …
WebbGao, R., Wang, Q., Li, P., Li, Z., & Feng, Y. (2016). Genome sequence and characteristics of plasmid pWH12, a variant of themcr-1-harbouring plasmid pHNSHP45, from ... WebbConclusion: This study reported a the multidrug-resistant Salmonella 4, [5],12:i:- isolate harboring mcr-1, blaCTX-M-14 and fosA3 from human for the first time in China. The occurrence of mcr-1 and fosA3 genes in the transferable IncHI2 plasmid pYZU1189 from the ESBL-producing Salmonella 4, [5],12:i:- isolate showed a potential threat to public ... sims 4 build mode commands
Colistin Resistance Gene mcr-1 Mediates Cell Permeability and ...
Webb27 juni 2024 · For instance, a 222.3-kb section of pWJ1 (85.4% of the total sequence) exhibited high similarity to the corresponding region of the 251-kb plasmid pHNSHP45-2 from E. coli strain SHP45, from which the first mcr-1 gene was identified . pWJ1 and pHNSHP45-2 had 12 resistance genes in common (Fig. 1A), while pWJ1 appeared to … WebbThe PCR based mapping of mcr-1 genetic context in our isolates revealed that almost all amplified genes were similar to pHNSHP45, except the truncated tnpA gene, missing IsApl1. Globally, plasmid analysis of various Incompatibility types has shown that IsApl1 is usually absent in IncX4 type plasmid while in Incl2 type plasmid, it is either absent or … Webb19 dec. 2016 · Compared with the original mcr-1-bearing IncHI2 plasmid pHNSHP45-2, 23 three regions in which antibiotic resistance genes were observable were found to exhibit structural differences (Figure S2). First, the IS Apl1 - mcr-1 - orf region located between terF and the hypothetical CDS was absent in pHYEC7-IncHI2, indicating that mcr-1 could … rbf13c2212a