Web1 apr. 2024 · The Procedure of Haploid-Inducer Mediated Genome Editing (IMGE) in Maize. A CRISPR/Cas9 cassette could be introgressed or directly transformed into a haploid … WebDoubled haploid technology using inducer lines carrying mutations in ZmPLA1/MTL/NLD and ZmDMP 1-4 has revolutionized traditional maize breeding. …
Review on Concept and Impact of Double Haploid Techniques in …
WebThe haploid inducer line KEMS ( Shatskaya et al., 1994) was used as a pollen donor and crossed with four simple hybrids (DKB393, GNS 3225, GNS 364, and GNS 3032) and their F2 generations. The cross field test occurred in Cravinhos, in the State of São Paulo (650 m altitude and 21°20ꞌ25ꞌS, 47°43ꞌ46ꞌW), in the second season of 2011. WebEmerging technologies using either stable or transient expression and tissue culture-independent methods, such as direct genome editing using RNA-guided endonuclease system as an in vivo desired-target mutator, simultaneous double haploid production and editing/haploid-inducer-mediated genome editing, and pollen transformation, are … insults deliberately
A DMP-triggered in vivo maternal haploid induction …
Web22 mrt. 2024 · Since different inducer lines have variable HIR learnt from maize, we expect that HIR can be improved by creating simtl lines with different genetic backgrounds or in … Webinducer lines carry the R1-nj gene, which causes a ‘‘red crown’’ endosperm as well as a red (or purple) embryo. After pollination by inducer lines, haploid seeds combine a haploid maternal embryo (colorless) and a triploid endosperm (red crown aleurone) derived from both, the maternal donor and the inducer line (Geiger 2009). However ... Web2 mei 2024 · A total of 16 lines were confirmed to have no exogenous T-DNA elements: five lines for line #1, two lines for line #2, two lines for line #3 and seven lines for line #4 ( … insults during a roast crossword clue