WebGPX4 (glutathione peroxidase 4), a downstream mediator of amino acid antiporter system xc −, is an antioxidant enzyme involved in reducing phospholipid hydroperoxides in membranes, thus conferring cytoprotection against ferroptosis [ 5 ]. The inhibition of GPX4 activity or the downregulation of GPX4 expression can induce ferroptosis [ 5–8 ]. WebGPX4 short hairpin RNA (shRNA) fragment was cloned into adeno-associated virus (AAV) vector GV478 (U6-MCS-CAG-EGFP) (Shanghai Genechem Co., Ltd) to construct AAV-shGpx4. AAV-293 cells were co-transfected with recombinant AAV-shGpx4, pAAV-RC and pHelper for AAV packaging.
Contribution of ferroptosis and GPX4’s dual functions to …
WebAug 12, 2024 · As a key regulator of ferroptosis, phospholipid peroxidase glutathione peroxidase 4 (GPX4) can detoxify hydroperoxides in membrane lipids directly, thereby reducing damage to membrane function... WebThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione … infobibos
GPX4 Inhibitor Screening Assay Kit - caymanchem.com
WebJul 15, 2024 · Given that GPX4 is a vital suppressor of ferroptosis, we wondered whether HSPCs undergo ferroptosis in the absence of GPX4. Hence, lineage − sca1 + c-kit + (LSK) cells isolated from the... WebApr 10, 2024 · Herein, we found that RUNX1-IT1 was capable of binding to IGF2BP1 protein, a stabilizer of mRNA via recognition of m 6 A motifs [ 30 ]. Silencing of IGF2BP1 blocked the increased GPX4 expression caused by RUNX1-IT1 overexpression, hinting that IGF2BP1 is required for RUNX1-IT1-mediated regulation of GPX4. WebJan 31, 2024 · The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly ... infobiker calendario