Fitc staining protocol
WebMar 19, 2024 · Throughout the whole staining procedure, tissue sections need to be protected from direct light exposure as fluorescently (e.g., FITC/TRITC-) labeled lectins quench more quickly than Alexa Fluor-labeled probes. Additionally, stained sections should be imaged within 4 days to ensure specific binding. WebFluorescence Microscopy Analysis of Annexin V-FITC Staining. HeLa cells were left untreated [top left panel] or induced to undergo apoptosis with camptothecin (8µM camptothecin, 24 h) [top middle panel]; or staurosporine (1µM, 4 h) [top right panel] and stained with Annexin V-FITC according to the protocol provided. bottom left panel, …
Fitc staining protocol
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WebBestProtocols: Viability Staining Protocol for Flow Cytometry ... Fluorescence microscope with FITC and TRITC filters; Protocol 1. Culture cells in appropriate medium and vessel for microscopy: 2. Thaw vials: 3. Transfer Live Green vial (A) into Dead Red vial (B) 4. Mix to create 2X stock WebFITC anti-human CD55 Antibody - CD55 is a 60-70 kD glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein also known as decay-accelerating factor (DAF). ... For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. ... Cell Surface ...
WebMar 19, 2024 · Throughout the whole staining procedure, tissue sections need to be protected from direct light exposure as fluorescently (e.g., FITC/TRITC-) labeled lectins … WebGeneral procedure for flow cytometry using a conjugated primary antibody. Print this protocol. Harvest, wash the cells, and adjust cell suspension to a concentration of 1-5 x 10 6 cells/mL in ice-cold PBS, 10% FCS, 1% …
WebApr 13, 2024 · Extracellular vesicles have shown good potential in disease treatments including ischemic injury such as myocardial infarction. However, the efficient production of highly active extracellular ... WebCD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70 and plays an important role in costimulation of T cell activation, and regulation of B ...
WebNov 9, 2024 · Stain cultured cells with phalloidin conjugates. Tip: Pre-incubating fixed cells with 1% BSA in PBS for 20–30 minutes may improve staining. Tip: When staining coverslips, keep them in a covered …
WebReagents and Protocol (adapted from Invitrogen): ... V-FITC and an antibody conjugated to APC. Because APC is excited by the Red (637 nm) laser, FITC is excited by the Blue (488 nm) laser, and PI ... Annexin V staining becomes detectable and then, as the cells become permeable, PI gains access to the DNA. References: how to snake a drain lineWebThe nuclei were counterstained with blue fluorescent Hoechst 33342 nucleic acid stain. The image was acquired using bandpass filter sets appropriate for the Texas Red dye, fluorescein, and DAPI. The image was acquired … novarc welding robotWebApplication Notes. Clone HI98 has been described to specifically react with 3-fucosyl-N-acetyllactosamine (3-FAL), also called X-hapten, SSEA-1, CD15, or Lewis X. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections 5 and formalin-fixed paraffin-embedded tissue sections. how to snake a drain toiletWebAvidin-FITC is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the recommended use of this reagent is … novare chemistry for accelerated studentsWebAntibody Protocols › Cell Separation Methods › FITC Protocols Fluorescein Isothiocyanate (FITC) - conjugated Antibodies GFP Protocols › Immunoprecipitation Protocols › Membrane Protein Protocols › Protein Expression Protocols › Protein … novare africa property fund iiWebWe recommend typical dilutions as follows: Imaging applications—1:500 to 1:1000; Flow cytometry— 1:2000 or higher. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. for the fluorophore- and biotin-labeled antibodies, including the phycoerythrin-, allophycocyanin-, and tandem … how to snake a kitchen sink pipeWebThe following protocol combines BrdU (FITC) staining of S phase cells in combination with propidium iodide for cell cycle analysis. This protocol is used courtesy of Prabhat … how to snake a kitchen sink youtube