Ea. hy926 cells
WebAug 5, 2015 · Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-dephenyltetrazolium bromide (MTT) assay, while the effect of TDD on the transcription profile of EA.hy926 cells subjected to H2O2-induced oxidative injury was evaluated by microarray analysis. Several signaling pathways, including apoptosis, were significantly … WebDec 30, 2013 · Because EA.hy926 cells do not express the CD138 surface marker, we further investigated CD138 expression on EA.hy926 cells to verify the interaction of the MM-MVs with the EA.hy926 cells.
Ea. hy926 cells
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WebMar 22, 2016 · A total of 150 μl of chilled Matrigel was added to a 48-well plate and incubated at 37 °C for 30 minutes. UC-MSCs, or EA.hy926 cells, or 1:1 mixed UC-MSC-EA.hy926 cells (total 35 × 10 3 cells per well) were suspended in 500 μl of growth media and were added to the solidified Matrigel. Additionally, EA.hy926 cells suspended in 500 … WebAug 22, 2016 · EA.hy926 cells were seeded on a collagen-coated polyethylene membrane insert (a pore size of 1.0 μm) in the Transwell TM chamber and challenged with DENV2 as aforementioned, but with 10 μg ...
WebMay 10, 2024 · Our results indicate that 1) EA.hy926 cells internalize B-ELNs in a dose-dependent manner; 2) pretreatment with B-ELNs counters TNF-α-induced reactive oxygen species (ROS) generation and loss of cell viability and modulates the differential expression of 29 genes (fold change > 1.5) induced by TNF-α compared to control; 3) pathway … WebPurpose: The aim of the study was to evaluate the effect of piperlongumine (2 and 4 µM) on endothelial EA.hy926 and lung adenocarcinoma A549 cells with regulated expression of …
WebMar 15, 2024 · EA.hy926 cells express very little RNA for caspase-5 and inflammasome sensors, NLRP3 and NLRC4, that are essential for caspase-1 activation . We stimulated … WebJul 21, 2015 · EA.hy926 cells were transduced with lentivirus-based shRNA targeting NS, STING (B), or cGAS (C). Cells were then infected with KSHV (30 DNA genome copies per cell) for 8 h before IFN-β mRNA was measured by real-time qPCR. The relative amount of IFN-β mRNA was normalized to the 18S ribosomal RNA level in each sample, and the …
WebEA.hy926 cells were treated with these gene complexes in the culture solution. EA.hy926 cells were treated with pEGFP-ZNF580 as a negative control group, and cells were treated with PEI 10 kDa/pDNA as a positive control group. The expression of GFP in transfected cells was observed under an inverted fluorescent microscope.
WebMar 31, 2024 · EA.hy926 cells, transfected with striatin siRNA or controls (untransfected), were treated with estrogen (5 × 10−8 mol/L), and mRNA levels of PTGIS (A) and PTGS1 (B) were determined. Results show no significant difference in the genomic response after 5 hours of estrogen stimulation in the transfected and control cells (*, P < .05 vs vehicle). inwear roseauWebIn the present study, we demonstrated that SLT could protect EA.hy926 cells from oxidative stress and cell damage caused by H 2 O 2. Furthermore, our results suggest that this effect is possibly mediated by a reduction of the Bax/Bcl-2 ratio and an increase of SOD activity in the EA.hy926 cells. inwear shirtWebMay 26, 2024 · EA.hy926 cells were seeded in 96-well plates at a concentration of 1 × 10 5 cells/mL and incubated at 37 °C under 5% CO 2 humidified conditions for 24 h. Once the plated cells reached 100% confluence, 10 μL of DAF-FM-DA (10 mM) was added and the culture was incubated for 30 min. The cell culture medium was then removed and … in wear tøjWebApr 13, 2024 · EA.hy926 cells within the logarithmic growth phase were seeded in 96-well plates (1 × 10 4 cells/cm 2) and incubated with different concentrations of phenolic acid (1, 5, or 25 μM) for 1 h. They were then separately co-incubated with TNF-α (10 ng/mL) or without TNF-α for 24 h. ELISA kits (Abcam, Cambridge, UK) were used to determine the ... only president to never have gotten marriedWebEA.hy926 endothelial cells were localized at the bottom of the cell insert and the alveolar type II A549 cell on the upper side (Figure 3a). Cells that resemble the round morphology of THP-1 were seen on top of the epithelial A549 cells ( Figure 3 b), which were recognized by the presence of small microvilli (arrows) and lamellar bodies (LM). in wear siriWebEA.hy926 endothelial cells were localized at the bottom of the cell insert and the alveolar type II A549 cell on the upper side (Figure 3a). Cells that resemble the round … inwear size chartWebSep 2, 2024 · The EA.hy926 cells, derived from the fusion of human umbilical vein endothelial cells (HUVECs) with a thioguanine-resistant clone of A549, were used as the … only president to serve three terms